A significant increase in the CD8+ T cell:Treg ratio was also observed in the spleen 7 days post-RFA (Fig 3D); however, this more favorable ratio was a consequence of an overall decrease in the proportion of Treg within the splenic compartment rather than an increase in CD8+ T cells as was observed in the tumor microenvironment (Fig 3C). This evidence concerns the gene CD8A and neoplasm.