PDGFB and bilateral striopallidodentate calcinosis: To study the functional consequences of PDGFB mutations described in PFBC (Fig 1A and 1B), cDNA sequences carrying the different mutations identified in six PFBC families were introduced into the pcDNA3.1 vector, transfected into human embryonic kidney (HEK) 293 cells, and after G-418 selection, stably expressing clones were generated.