To study the functional consequences of PDGFRB mutations, pcDNA3.1 constructs containing 3 different PDGFRB mutations identified in PFBC patients (L658P, R987W and E1071V, Fig 1C) were transfected into porcine aortic endothelial (PAE) cells. Here, PDGFRB is linked to bilateral striopallidodentate calcinosis.