In an effort to profile the expression pattern of TRIM superfamily in several NSCLC cell lines, the expression of several TRIM genes including TRIM3, TRIM7, TRIM14, TRIM16, TRIM21, TRIM22, TRIM29, TRIM59, TRIM66 and TRIM70 was significantly upregulated in NSCLC cell lines compared with normal human bronchial epithelial (HBE) cell line, whereas the expression of other TRIM genes including TRIM4, TRIM9, TRIM36, TRIM46, TRIM54, TRIM67 and TRIM76 was significantly down-regulated in NSCLC cell lines compared with that in HBE cells. The gene discussed is TRIM3; the disease is non-small cell lung carcinoma.