In fact, RP is usually prescribed as decoctions such as “Kai Xin San” and “Ding Zhi Xiao Wan” in traditional Chinese medicine24, 25, this prompts us to investigate the pharmacological and mechanistic actions of RP. Our previous study has suggested that onjisaponin B isolated from RP, induces autophagy and attenuates the protein level of mutant proteins including α-synuclein and huntingtin, which are highly associated with HD and PD respectively21. This evidence concerns the gene HTT and Huntington disease.