Notably, as shown in Figure 1A, PBLD mRNA expression was significantly decreased in HCC tissues compared with that in adjacent non-tumorous tissues, which was detected in 84.3% (91/108) of HCC patients, and PBLD mRNA expression in moderate to poor-differentiated HCC tissues was more frequently downregulated than that in well-differentiated tissues (P = 0.002). This evidence concerns the gene PBLD and hepatocellular carcinoma.