Furthermore, using yeast two-hybrid, pulldown and co-immunoprecipitation assays, we showed that FlnA-G288R and P637Q mutations and a recently-identified mutation p.His743Pro (H743P) affect FlnA/PTPN12 interactions, supporting a potential role of altered FlnA/PTPN12 interactions in the physio-pathological mechanisms of FlnA-associated MVP. This evidence concerns the gene PTPN12 and familial mitral valve prolapse.