The data from the qRT-PCR analysis also validated the down-regulated expression of the complement components C1s-like, C2, C3, C4, C5, C8A and C9 in response to the GSIV infection, however the weak up-regulated expression of C1r, C1s and C7 in the transcriptome analysis could not be validated by qRT-PCR. The gene discussed is C3; the disease is infection.