CHD8 and acute lymphoblastic leukemia: When transduced into B-ALL cells, TRMPVIR-shChd8-1 leads to a marked reduction of CHD8 expression by 12 hours following treatment with doxycycline, while CHD8 levels in cells transduced with TRMPVIR expressing a Renilla luciferase shRNA (shRen) are not affected (Fig 3B).