Classically and alternatively activated macrophages (M1 and M2, respectively) have been reported to display opposing functions during retinal degeneration.27, 28 Ccr2 and Ly6c are considered as M1 markers, while Cx3cr1 is a M2 marker.27, 28, 29 To determine the macrophage subtypes within the subretinal space following detachment of the retina, we evaluated Ccr2, Ly6c, and Cx3cr1 mRNA expression in macrophage/microglia cells isolated on days 1 and 7 via laser capture microdissection (LCM) followed by quantitative real-time PCR (qPCR). The gene discussed is CX3CR1; the disease is retinal degeneration.