Even though the effector function of the LAP TGF-β subset of the CD4+CD25+CD127− Treg cells was not directly evaluated in our study, Mahalingam et al. and Scurr et al. reported that peripheral blood and tumor-infiltrating LAP+ Treg cells exhibit potent suppressive activity in colorectal cancer [20, 21]. This evidence concerns the gene CD4 and neoplasm.