Since Gpnmb is predominantly expressed in SVF of WATs in C57BL/6JJcl mice fed with HFHS chow, we generated Gpnmb−/− mice by backcrossing DBA2J mice naturally occurring stop codon mutation of Gpnmb (R150X) to C57BL/6JJcl mice in order to explore the role of Gpnmb in obesity and metabolic syndrome. Here, GPNMB is linked to obesity due to melanocortin 4 receptor deficiency.