On the other hand, the gene splicing of XBP1u (XBP1s) (Fig. 4F) and protein expression of p-IRE1α (Fig. 4G) was transiently induced at 17 wks (cohort2), but not at 22 wks (Fig. 6F,G) (cohort 3); however, TUDCA treatment significantly suppressed gene splicing of XBP1u (XBP1s) only in CR pups (CR-Veh v.s. CR-TU), but not in AD pups (AD-Veh v.s. AD-TU) (Fig. 6F) (cohort 3), suggesting a potential involvement of XBP1s in the deterioration of hepatic steatosis between 17 wks and 22 wks. This evidence concerns the gene ERN1 and Alzheimer disease.