IFNB1 and Stroke: To study the potential involvement of IFN-β in the regulation of peripheral immune cell subsets post-stroke, we isolated peripheral blood mononuclear cells (PBMCs) 2 days after tMCAo, as well as 8 days following tMCAo and sham surgery, and we quantified different immune cell subsets using flow cytometry (see Fig. 1a for the experimental outline).