Our Western blot analysis of cell lysates revealed the global levels of EZH2-mediated H3K27me3 and H1.2 much higher in MCF7 breast, LD611 bladder and LNCaP prostate cancer cells than in their nontransformed cells (MCF10-2A, LD419, and MLC) (Fig. S3A). This evidence concerns the gene EZH2 and prostate carcinoma.