The comparison of Relative Solvent Accessible Surface Area and Relative Δ Gprotein appeared to be a decisive parameter to separate out mutant population from the stable population obtained for WT at 300 K. Based on all these parameters, the QM-MM simulations reported here were able to clearly separate out the temperature dependent properties of V143A, R249S and R175H in comparison to wild type at 300 K and 310 K. These structural aspects may be considered for designing rescue strategies for the p53 cancer mutants. The gene discussed is TP53; the disease is cancer.