As shown in Figure 3, the proinflammatory Th1/Th17 cytokines IFN-γ (Figure 3B) and IL-17A (Figure 3C) transcripts were expressed at high levels in the CD4+CD25− subset of IBD model mice, whereas the natural regulatory T cell (Treg) markers CD25 (Figure 3D), IL-10 (Figure 3E), and Foxp3 (not shown) were predominantly expressed in the CD4+CD25+ subset. This evidence concerns the gene CD4 and inflammatory bowel disease.