The results of this study suggest that further studies including, assessment of glucose; insulin and leptin level; pre-treatment in vivo and deferent concentration of metformin; and spindle assembly detection are needed to evaluate the metabolic features and effects of metformin on oocyte at the molecular and cellular levels throughout the IVM of oocytes retrieved from EV PCOS-induced rat. Here, LEP is linked to polycystic ovary syndrome.