AKAP13 and infection: All mice infected with wild type or Δasp5CRISPR:ASP5WT-HA3 tachyzoites succumbed to infection by day 10, whereas those injected with Δasp5CRISPR parasites exhibited a delay to death, including one mouse that survived the experiment and was seropositive for anti-Toxoplasma antibodies when tested at day 14 (Figure 10—figure supplement 1B).