PKM and cholangitis: The mean ± SD intensity of PKM2 staining increased with the progression of HC: 0.36 ± 0.48 for cholangitis, 0 for hyperplasia and stage I disease, 1.11 ± 1.11 for stage II disease, 1.6 ± 1.26 for stage III disease, and 2.00 ± 1.25 for stage IV disease (Fig. 2b).