To examine whether the rs2853669 variant (−245T > C) affects E2F1-mediated DNMT1 and HDAC1-binding to the TERT promoter, we analyzed the interaction between E2F1 and the rs2853669 variant in HepG2 cells, which is a HCC cell line with the rs2853669 variant (Supplementary Figure 3). Here, DNMT1 is linked to hepatocellular carcinoma.