Moreover, similar to the FGF2 signaling inhibition effects observed in vitro, formononetin also significant decrease PI3K, Akt, MMP-2, MMP-9, and STAT3 in MDA-MB-231 tumor sections according to western blot, further demonstrating that formononetin played an important role in suppressing angiogenesis at least in part via FGFR2 signaling pathway. This evidence concerns the gene MMP9 and neoplasm.