To further investigate the correlation between SETD2 mutations and transcription read-through in ccRCC, we performed RNA sequencing of 2 SETD2 wild type (wt) and 4 SETD2 mutant ccRCC cell lines previously reported to have a marked reduction of H3K36me3 levels (Duns et al., 2010; Carvalho et al., 2014). Here, SETD2 is linked to nonpapillary renal cell carcinoma.