Of the 9 genes tested, 8 (THBS1, CYR61, CTGF, MXRA5, SPP1, LTBP2, TGFBR1 and COL11A1) were found by qRT-PCR to be significantly differentially expressed in tumor-associated fibroblasts, for a validation rate of 89%. The gene discussed is SPP1; the disease is neoplasm.