As to the protein concentration, HE4-H group obtained the most highest HE4 concentrations both in tumor tissues and blood by ELISA quantification, with values of 214.5 ± 78.2 ng/mL and 239.2 ± 67.5 ng/mL, moreover, HE4-L group gained the most lowest concentrations, with values of 18.2 ± 9.1 ng/mL and 17.5 ± 15.3 ng/mL, compared with the mock group 96.8 ± 37.8 ng/mL and 90.2 ± 29.8 ng/mL, respectively (P < 0.05, Figure 3C). Here, WFDC2 is linked to neoplasm.