Although PRC2 target genes were both upregulated (n=125) and downregulated (n=70; P<0.05; t-test, one-tailed, unequal variance) in CCSKs (Fig. 3c and Supplementary Data 5) relative to Wilms tumours, analysis using GSEA and the Database for Annotation, Visualization and Integrated Discovery (DAVID)27, 28 toolset also revealed a highly significant enrichment for homeobox proteins (FWER P<0.001; Supplementary Fig. 7) and upregulation of distinct classes of homeobox proteins, which are the canonical targets of polycomb-mediated repression. Here, POU2F2 is linked to Wilms tumor.