By quantitative PCR we found that indeed the expression of important cytokines Cd54, Csf-1, Cxcl1, Cxcl2 and Ccl2 was significantly increased in 2cKO mouse HNSCC tumor lysates as compared with 2cKO mouse tongue and the control tongue (Fig. S5: n = 5 respectively). This evidence concerns the gene CCL2 and neoplasm.