To study the role of zebrafish MEK2 in the formation of skin tumors and comparison of expression with MEK1, pTol2-krt14-MEK2-GFP, pTol2-MEK2S219D-GFP, pTol2-MEK2S223A-GFP, pTol2-MEK1-mCherry and pTol2-MEK1S219D-GFP were microinjected into one-cell stage of zebrafish embryos and revealed the expression at 3 dpf. This evidence concerns the gene MAP2K2 and skin neoplasm.