PIK3CA, EGFR, HRAS, MYC, CDKN2A, MET, TRAF2, PTK2 and CASP8. Of the novel genes, JAK1 was found to be amplified in two of the cell lines and overexpressed in all 4 HNSCC cells; NOTCH1 known to harbor inactivating mutations in HNSCC [3, 50] was found to be amplified in all 4 and overexpressed in 2 of 4 HNSCC cells, known to be play dual role in a context dependent manner [59]. This evidence concerns the gene NOTCH1 and head and neck squamous cell carcinoma.