Also the allosteric AKT inhibitor, MK2206, inhibited cell proliferation (Fig 5E) in primary cultures of mucin-IHCCA (IC50 = 4.5 ± 2.3 μM) and mixed-CCA (IC50 5.6 ± 0.53 μM) but at a significant lower extent in comparison with NVP-BEZ235. This evidence concerns the gene AKT1 and cholangiocarcinoma.