We explored the effects of Kv1.5 silencing on proliferation and growth of osteosarcoma cells using CCK-8 and colony formation assays, and we found that Kv1.5 silencing significantly suppressed cell proliferation and growth after a 48-h treatment compared to the control-shRNA or untreated groups in MG-63 cells. This evidence concerns the gene KCNA5 and osteosarcoma.