ACR and tuberculosis: We chose mycobacteria-specific antigens based on (1) proteome analysis of antibody responses to Mtb from active TB patients (included Acr, Ag85, CFP-10, and HrpA) [25, 26]; (2) in silico studies performed to identify TB vaccine candidates (included Acr, Ag85, and ESAT-6) [27]; and (3) publications identifying HBHA and MDP1 as mycobacteria-specific antigens [22, 28, 29].