DDB1 and infection: To further validate the function of UBE2A and DDB1 in the DENV life cycle, we tested if silencing of these two genes would also reduce plaque titers without altering RNA replication in the head/thorax of Ae. aegypti. Mosquitoes were infected via intra-thoracic inoculation to ensure that all mosquitoes have disseminated infections by bypassing the midgut.