To identify novel drivers of NKG2D ligand expression, we performed a genome-wide loss-of-expression mutant screen in the tumor-derived human cell line HAP1 (Carette et al., 2009; Carette et al., 2011) and used CRISPR/Cas9 gene targeting methodology for confirmation of the hits and extension of the results. This evidence concerns the gene KLRK1 and neoplasm.