SPP1 overexpression in cancer cells led to the down-regulation of E-Cadherin and up-regulation of N-Cadherin and Slug at both the mRNA and protein levels (Figure 6—figure supplement 1J,K), and up-regulation of Vimentin and Snail at the mRNA level (Figure 6—figure supplement 1J). This evidence concerns the gene CDH2 and cancer.