TNF and Alzheimer disease: To determine whether the cytokines identified from our regression profile could potentially drive AD pathogenesis, we assessed viability of primary mouse neuron cultures treated with combinations of Aβ, the top three up-regulated cytokines from the EC/SFG ratio analysis (VEGF, TNF-α IL-5), and IL-12p70, which is among the top 11 cytokines identified in all regions Fig. 1c and Supplementary Fig. S4b,d and has been implicated in AD38.