In this study, we found that β-elemene reduced the invasiveness of glioblastoma cells in vitro and further showed that β-elemene inhibited EMT by upregulating the expression levels of E-cadherin and downregulating the expression levels of N-cadherin and β-catenin in glioblastoma cells in vivo and in vitro. This evidence concerns the gene CDH1 and glioblastoma.