To assess if the IFNγ antiviral effect on macrophages was independent of type I IFN receptor signaling, peritoneal macrophages were harvested from interferon α/β receptor knockout mice (IFNAR-/-) and cultured for 24 hours with M-CSF or M-CSF plus IFNγ prior to EBOV GP/rVSV infection. This evidence concerns the gene IFNAR1 and infection.