Analysis of BCR-ABL expression by qPCR and Western blot demonstrated that BCR-ABL mRNA and protein, including phosphorylated protein (p-BCR-ABL) were present in undifferentiated CML iPSCs and their iCD34+ and iCD34− progeny (Fig. 3a and b). This evidence concerns the gene ABL1 and chronic myelogenous leukemia, BCR-ABL1 positive.