To investigate DNA methylation changes in the earliest phase of breast cancer malignancy, Susan Clark’s group used an in vitro system that recapitulates the first stages of basal-like breast cancer and performed MBDCap-sequencing (affinity capture of methylated DNA with recombinant methyl-CpG binding domain of MBD2 protein followed by next generation sequencing) [75]. Here, MBD2 is linked to breast cancer.