AD WT infection, but not AD ORF1 D68A infection (MYC activation-deficient), increases the percentage of intracellular M5 isotopologue-labelled (five carbon-labelled) glutamate relative to mock-infected cells, consistent with a MYC activation-dependent increase in GLS-catalysed conversion of glutamine to glutamate during adenovirus infection (Supplementary Fig. 1b,c). Here, GLS is linked to adenoviridae infectious disease.