To determine whether sPRDM16 played a role in the maturation of AML cells, we incubated THP-1 cells with 3 nM PMA for 24 h, and measured cell-surface expression of the monocytic maturation marker CD11b using APC-labeled anti-CD11b antibody by flow cytometry (Fig. 1f). Here, ITGAM is linked to acute myeloid leukemia.