However, U251 cells exposed to different concentrations (25–100 μg/mL) of CCF for 24 h displayed necrotic cell death, which was mainly due to the necrosis population observed by Annexin V/PI assay (Figure 4(a)), while CCF-induced later apoptosis was the main cell death observed in DBTRG-05MG cells, and 0.1% DMSO controls showed no significant necrosis or apoptosis in the different glioblastoma cells. Here, ANXA5 is linked to glioblastoma.