Western blot assays showed that rhPOSTN apparently increased phosphorylation of Akt (pAkt) in SW480 and HT29 cells and expression level of β-catenin in HT29 cells; whereas 10 μM LY294002 or XAV939 apparently reduced rhPOSTN-upregulated expression of pAkt and β-catenin in CRC cells (Figure 4H). The gene discussed is AKT1; the disease is colorectal carcinoma.