The present study aimed to investigate the p53 gene mediated expression of miRNAs, cyto- and chemokines in human colon cancer cells (HCT116) after the treatments of radiation and SN38, and further examined the most significantly up- or down-regulated miRNAs to find out whether there is any possible interaction between these miRNAs and increased or decreased cyto- and chemokine expression in colon cancer cells in response to the radiation and SN38 treatments. This evidence concerns the gene TP53 and malignant colon neoplasm.