To test possible associations between MSX1 and CHD, we conducted SNP analyses and found that both rs3821949 and rs12532 were associated with the risk of CHD (specifically VSD) in Chinese Han population (Tables 2 and 3).The Hardy-Weinberg equilibrium test for the CHD and controls were conducted and it was in line with the equilibrium. The gene discussed is MSX1; the disease is ventricular septal defect.