To better understand the prognostic role of FGFR1 amplification in Caucasian patients, we employed fluorescence in-situ hybridization (FISH) analysis for precise determination of the FGFR1 amplification rate in a large tissue microarray made from 254 ESCC and 346 EADC patients with histopathological and clinical follow-up data of Caucasian origin. This evidence concerns the gene FGFR1 and esophageal squamous cell carcinoma.