To assess whether AR-V7 could mediate resistance to an AR antagonist in breast cancer, we transiently transduced MDA-MB-453 cells with a lentivirus designed to over-express AR-V7 (Supplementary Figure S10) and assessed cell growth in response to treatment with a contemporary AR antagonist, enzalutamide, using Crystal Violet assays. The gene discussed is AR; the disease is breast cancer.