In the present study, we further examined the relationship between EMT and IGF1R expression with sensitivity to EGFR-TKIs in NSCLC cell lines with wild-type or mutant EGFR. Furthermore, using in vitro assays, we provided evidence that IGF1R induced EGFR-TKIs resistance by inducing EMT and explored the possible cellular mechanism. This evidence concerns the gene IGF1R and non-small cell lung carcinoma.