To verify that the behavioural effects of 5XFAD/AEP−/− were not strain dependent, we also examined the rescue effect of AEP gene knockout in another well-established AD mouse model expressing two AD mutant genes, APP and PS1. APP/PS1/AEP−/− mice at 15 months of age showed shorter latency periods and swim path distance than APP/PS1 mice during the training sessions, and spent more time in the target quadrant during the probe test (Supplementary Fig. 11a–d). This evidence concerns the gene PSEN1 and Alzheimer disease.