HIF1A and melanoma: Because of the potential for off target pro-oxidant effects with the use of high concentrations of ascorbic acid (mM concentrations), our studies aimed to determine whether physiologically attainable serum concentrations of AA [35, 36], which would be achievable through the consumption of vitamin C rich foods (yielding up to 100 μM serum AA) or oral dietary supplements (up to 250 μM serum AA), could effectively impact HIF-1α in melanoma cells [36].